Comparative iTRAQ proteome and transcriptome analyses of sweet orange infected by “Candidatus Liberibacter asiaticus”
Identifieur interne : 001892 ( Main/Exploration ); précédent : 001891; suivant : 001893Comparative iTRAQ proteome and transcriptome analyses of sweet orange infected by “Candidatus Liberibacter asiaticus”
Auteurs : Jing Fan [République populaire de Chine, États-Unis] ; Chunxian Chen [États-Unis] ; Qibin Yu [États-Unis] ; Ronald H. Brlansky [États-Unis] ; Zheng-Guo Li [République populaire de Chine] ; Frederick G. Gmitter Jr [États-Unis]Source :
- Physiologia Plantarum [ 0031-9317 ] ; 2011-11.
English descriptors
- KwdEn :
- Chitinase (metabolism), Citrus sinensis (genetics), Copper (metabolism), DNA, Bacterial (analysis), Gene Expression Profiling, Gene Expression Regulation, Plant, Lipoxygenase (metabolism), Plant Diseases (genetics), Plant Diseases (microbiology), Plant Leaves (chemistry), Proteobacteria (physiology), Proteome, Superoxide Dismutase (metabolism), Zinc (metabolism).
- MESH :
- chemical , analysis : DNA, Bacterial.
- chemical , metabolism : Chitinase, Copper, Lipoxygenase, Superoxide Dismutase, Zinc.
- chemistry : Plant Leaves.
- genetics : Citrus sinensis, Plant Diseases.
- microbiology : Plant Diseases.
- physiology : Proteobacteria.
- Gene Expression Profiling, Gene Expression Regulation, Plant, Proteome.
Abstract
Citrus Huanglongbing (HLB) has been threatening citrus production worldwide. In this study, a comparative proteomic approach was applied to understand the pathogenic process of HLB in affected sweet orange leaves. Using the isobaric tags for relative and absolute quantification (iTRAQ) technique, we identified 686 unique proteins in the mature leaves of both mock‐inoculated and diseased ‘Madam Vinous' sweet orange plants. Of the identified proteins, 20 and 10 were differentially expressed in leaves with and without symptoms of HLB (fold change > 2.5), respectively, compared with mock‐inoculated controls. Most significantly, upregulated proteins were involved in stress/defense response, such as four miraculin‐like proteins, chitinase, Cu/Zn superoxide dismutase and lipoxygenase. Microarray analysis also showed that stress‐related genes were significantly upregulated at the transcriptional level. For example, remarkable upregulations of miraculin‐like proteins and Cu/Zn superoxide dismutase transcripts were observed. Moreover, the transcriptional patterns of miraculin‐like protein 1 and Cu/Zn superoxide dismutase were examined at different stages of HLB disease development. Combined with the transcriptomic data, the proteomic data can provide an enhanced understanding of citrus stress/defense responses to HLB.
Url:
DOI: 10.1111/j.1399-3054.2011.01502.x
Affiliations:
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In this study, a comparative proteomic approach was applied to understand the pathogenic process of HLB in affected sweet orange leaves. Using the isobaric tags for relative and absolute quantification (iTRAQ) technique, we identified 686 unique proteins in the mature leaves of both mock‐inoculated and diseased ‘Madam Vinous' sweet orange plants. Of the identified proteins, 20 and 10 were differentially expressed in leaves with and without symptoms of HLB (fold change > 2.5), respectively, compared with mock‐inoculated controls. Most significantly, upregulated proteins were involved in stress/defense response, such as four miraculin‐like proteins, chitinase, Cu/Zn superoxide dismutase and lipoxygenase. Microarray analysis also showed that stress‐related genes were significantly upregulated at the transcriptional level. For example, remarkable upregulations of miraculin‐like proteins and Cu/Zn superoxide dismutase transcripts were observed. Moreover, the transcriptional patterns of miraculin‐like protein 1 and Cu/Zn superoxide dismutase were examined at different stages of HLB disease development. Combined with the transcriptomic data, the proteomic data can provide an enhanced understanding of citrus stress/defense responses to HLB.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Fan, Jing" sort="Fan, Jing" uniqKey="Fan J" first="Jing" last="Fan">Jing Fan</name></noRegion><name sortKey="Li, Zheng Uo" sort="Li, Zheng Uo" uniqKey="Li Z" first="Zheng-Guo" last="Li">Zheng-Guo Li</name></country><country name="États-Unis"><region name="Floride"><name sortKey="Fan, Jing" sort="Fan, Jing" uniqKey="Fan J" first="Jing" last="Fan">Jing Fan</name></region><name sortKey="Brlansky, Ronald H" sort="Brlansky, Ronald H" uniqKey="Brlansky R" first="Ronald H." last="Brlansky">Ronald H. Brlansky</name><name sortKey="Chen, Chunxian" sort="Chen, Chunxian" uniqKey="Chen C" first="Chunxian" last="Chen">Chunxian Chen</name><name sortKey="Gmitter Jr, Frederick G" sort="Gmitter Jr, Frederick G" uniqKey="Gmitter Jr F" first="Frederick G." last="Gmitter Jr">Frederick G. Gmitter Jr</name><name sortKey="Yu, Qibin" sort="Yu, Qibin" uniqKey="Yu Q" first="Qibin" last="Yu">Qibin Yu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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